A simple R-banding technique by BrdU-Hoechst treatment and Giemsa staining following heating and ultraviolet exposure
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چکیده
منابع مشابه
Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
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A 21-year-old man presented with a painless ulcer on the penis, which had been present for 4 weeks, and diffuse, symmetrical erythematous maculopapules on the trunk, extremities, palms, and soles, which had been present for 1 week. Gram staining of bloody exudate from his chancre revealed very weakly Gram-negative spiral rods (Picture 1; × 1,000). We therefore performed Giemsa staining, which r...
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ژورنال
عنوان ژورنال: Japanese journal of human genetics
سال: 1984
ISSN: 0021-5074
DOI: 10.1007/bf01873534